DH5a Electro Competent Cells

DH5α Electrocompetent Cells are high-efficiency E. coli cells optimized for electroporation-based DNA transformation, offering superior plasmid yield, stability, and cloning accuracy. The DH5α strain carries recA1 and endA1 mutations to minimize recombination and nuclease degradation, ensuring reliable propagation of high-quality plasmid DNA. These cells routinely achieve transformation efficiencies up to 1x1010 cfu/μg pUC19 DNA under standard electroporation conditions (1.8 kV, 1 mm cuvette) . Ideal for library construction, subcloning, plasmid rescue, and large construct propagation. Supplied as single-use aliquots ready for electroporation, compatible with all major electroporators. Each lot is QC-tested for efficiency, sterility, and genotype confirmation.