Tissue RNA Mini Kit with Dnase I Set

The Tissue RNA Mini Kit with DNase I Set provides a fast, efficient method for isolating high-quality total RNA from animal or tissue samples (~≤25 mg tissue or equivalent cells) . The kit includes a robust denaturing lysis buffer to rapidly inactivate RNases, a spin-column or glass-fiber membrane binding format, optional on-column DNase I treatment to remove genomic DNA contamination, wash steps to remove proteins and inhibitors, and elution of clean RNA in RNase-free water. Typical yields range from ~5–30 μg RNA (depending on tissue type and amount) . The purified RNA is suitable for RT-PCR, Northern blotting, microarray analysis and RNA-seq workflows. The set contains lysis buffer, binding columns, wash buffers, elution buffer, RNase-free micropestles for homogenization, DNase I enzyme with reaction buffer, collection tubes and a detailed user manual. No phenol-chloroform extraction or ethanol precipitation required.