Express Endofree Plasmid Maxiprep

The Express EndoFree Plasmid Maxiprep Kit from BioMiga offers a rapid, high-yield plasmid DNA purification workflow with built-in endotoxin removal—designed for 100–200 mL of E. coli culture and yielding up to ~1 mg of plasmid DNA (high-copy) per prep. Utilizing BioMiga’s proprietary ezBindTM silica-matrix and the specialized Buffer RET system, the kit efficiently binds DNA while removing endotoxin, proteins and other contaminants, achieving endotoxin levels below <0.1 EU/μg DNA. The buffers are free of guanidine salts and ion-exchange resin residues, ensuring compatibility with sensitive downstream applications such as transfection of primary or endotoxin-sensitive cells, microinjection, gene therapy vector preparation and sequencing workflows. The streamlined protocol completes in approximately 30–40 minutes, featuring lysate clarification via filter syringe, DNA binding, washing and high-yield elution. The kit includes ezBindTM maxicolumns, Buffer A1, B1, N3, Buffer RET, EndoFree Elution Buffer, RNase A, and a detailed protocol—ideal for molecular biology, biotech and therapeutic-research settings where speed, purity and endotoxin‐free DNA are critical.