Express Endofree Plasmid Midiprep

The Express EndoFree Plasmid Midiprep Kit from BioMiga delivers a rapid, high-quality plasmid purification solution with endotoxin-reduction, designed to process 15–50 mL of E. coli culture and yield up to ~250 μg of plasmid DNA per preparation. The kit combines BioMiga’s proprietary ezBindTM silica-matrix with the specialized EndoFree buffer system (Buffer RET) to efficiently bind DNA while removing endotoxins, proteins and other contaminants. The resulting DNA is free of guanidine salts and ion-exchange resin residues, ensuring compatibility with sensitive downstream applications such as primary-cell transfection, microinjection, sequencing or library screening. Key features include syringe-filter lysate clarification (no high-speed floor centrifuge required), rapid binding/wash/elution workflow (~30 min), and components tailored for endotoxin-sensitive workflows. The kit includes ezBindTM midi columns, filter syringes, Buffer A1, B1, N3, RET, DNA Wash Buffer, RNase A, EndoFree Elution Buffer and a detailed user manual. Ideal for molecular biology research, gene therapy vector preparation and high-demand plasmid workflows.