EzFlow Endofree Plasmid Megaprep 6mg

The EndoFree Plasmid ezFlow Megaprep 6 mg Kit from BioMiga is a high-capacity, endotoxin-reduced plasmid DNA purification solution engineered for large-scale preparations—capable of purifying up to ~6 mg of high-copy plasmid DNA from ~1 L of E. coli culture within ~60 minutes. Leveraging BioMiga’s proprietary ezBindTM column matrix, and the EZgeneTM EndoFree buffer system, the kit reliably reduces endotoxin levels to <0.2 EU per μg DNA. The protocol is guanidine-salt and ion-exchange resin-free, enabling downstream applications such as transfection of primary or endotoxin-sensitive cells, DNA vaccination, and gene-therapy vector production. Workflow includes large-volume alkaline lysis, dual-layer filtration for rapid lysate clarification, binding, washing and high-yield elution. Kit components include pre-packed ezBindTM Megacolumns, large-volume lysis and wash buffers, endotoxin-removal reagents, RNase A, and detailed protocol. Ideal for high-demand plasmid production in molecular biology, biotech, and therapeutic research settings.