EzFlow Endofree Plasmid Maxiprep

The EndoFree Plasmid ezFlow Maxi Kit from BioMiga offers a rapid, high-yield solution for isolating endotoxin-free plasmid DNA from 150–200 mL (up to 250 mL) of E. coli culture. Leveraging BioMiga’s proprietary ezBindTM column matrix and EZgeneTM EndoFree buffers, this silica-based system binds DNA with high efficiency while eliminating chaotropic salts and guanidine/anion-exchange resin residues. The specialized endotoxin-removal buffers reduce endotoxin to <0.1 EU per μg of plasmid DNA, ensuring suitability for sensitive downstream applications such as transfection of primary or endotoxin-sensitive cells, microinjection, sequencing or library screening. The kit supports yields up to ~1 mg plasmid DNA (from high-copy plasmids) and is optimized for downstream use without further purification. Included are ezBindTM maxi columns, Buffer A1, B1, N3, Buffer RET (endotoxin removal), Elution Buffer, RNase A, plus user manual. Designed for fast cycling (less than 60 minutes) and reliable performance, this kit is ideal for molecular biology, gene therapy research and high-demand plasmid preparations.