α-Synuclein polyclonal antibody

Synthetic peptide corresponding to [Cys] + aa 117-131 of human α-synuclein conjugated to KLH. The sequence is conserved in rat α-synuclein except for two substitutions, namely Asp121Ser and Asn122Ser.

Vial contents, Storage and Use: Vial contains an immunoglobulin preparation, partially purified by chromatography from whole serum, suspended in phosphate-buffered saline (pH 7.4) containing 0.01M sodium azide. Store unopened vial at -20°C until required for use. AVOID REPEATED FREEZE-THAW CYCLES. Aliquot undiluted antibody into smaller volumes (not less than 10μL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used) .

Western blot (1:1,000-1:20,000) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

This antibody has been shown to react strongly with human brain-derived α-synuclein. The antibody also reacts with rat brain-derived α-synuclein but reactivity appears substantially less than with human.

Western blot: The antibody has been characterized by single dimension SDS-PAGE using recombinant α- and β-synucleins as well as rat, mouse and human brain lysates with and without peptide blockade. Studies with the recombinant proteins demonstrated the absolute specificity of the antibody for α-synuclein. The antibody gives a strong band at a relative molecular weight of approximately 15kDa with human brain lysate which is wholly abolished by preincubation with the immunizing peptide (Prod. No. BML-SP9340) . Immunohistochemistry: The antibody has been tested on de-waxed tissue sections of human brain fixed in neutral buffered formaldehyde. A standard protocol should be followed.