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WESTERNVIEW®Dual Detection Kit (Anti-Mouse/Anti-Rabbit)

Product Specifications

Certification

RUO

Species Reactivity

Mouse,Rabbit

Applications

WB

Assay Performance Time

2 hours

Shipping Conditions

Blue Ice

Storage Conditions

+4°C

Contents

Antibody Diluent, 4 x 90 mL Diluent solution for dilution of primary and secondary antibodies. NBT/BCIP, Ready-to-Use, 100 mL Ready-made solution for chromogenic blotting to be used when developing anti-rabbit secondary. DAB Chromogen (25X), 4 mL Concentrated chromogen for chromogenic blotting to be used when developing anti-mouse secondary. DAB Substrate Buffer, 100 mL Buffer used to dilute DAB Chromogen (25X) . SignaSure ® Buffer Salts, 2 packets Contents used to make SignaSure® Wash Buffer. Tween ® -20, 2 mL Detergent for SignaSure ® Wash Buffer. Secondary Antibody (HRP Anti-Mouse), 2 mL Anti-Mouse secondary antibody for detection of mouse-based primary antibodies in Western Blots. Use DAB Substrate Buffer and Chromogen to develop. Secondary Antibody (AP Anti-Rabbit), 2 mL Anti-Rabbit secondary antibody for detection of rabbit-based primary antibodies in Western Blots. Use NBT/BCIP solution to develop.

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