VEGFR2 (human), (recombinant) (GST-tag)
Product Specifications
Specifications
≥400 pmol/min/μg. Assay was done in Kinase buffer containing 0.2 mM DTT using Poly- (Glu4:Tyr) -biotin substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega) .
Product Name Alternative
KDR, FLK-1, Vascular endothelial growth factor receptor 2
Certification
RUO
UniProt
P35968
Source
Produced in Sf9 insect cells with N-terminal GST tage. Human VEGFR2 (aa 805-1356) . Produced in a baculovirus expression system.
Stability
When stored under the above conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing, could lead to loss of activity.
Purity
≥90% (SDS-PAGE)
Molecular Weight
~87 kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 40 mM TRIS, pH 8.0, containing 110 mM NaCl, 2.2 mM KCl, 0.02% Tween-10, 20% glycerol, and 3 mM DTT.
Applications Notes
Useful tool to study enzyme regulation and kinetics, phosphorylate target substrates, screen for inhibitors.
GenBank Number
AF035121
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