UDP-6-azide-glucose

Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 ß-Glycosyltransferase (T4 β-GT) . The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu (I) -free click chemistry that offers the choice to introduce a (Desthio) Biotin group for subsequent purification tasks (via DBCO-containing (Desthio) Biotin) to introduce fluorescent group for subsequent microscopic imaging (via DBCO-containing fluorescent dyes) . 0.5 mg UDP-6-azide-glucose are sufficient to prepare 283 μl of a 3 mM solution.