UCH-L3 (human), (recombinant) (His-tag)
Product Specifications
Specifications
>1000 pmol/min/μg. Determined at 25°C with 1μM ubiquitin-AMC (Prod. No. BML-SE211) as substrate and UCH-L3 at 20pM (0.58ng/ml; see protocol) .
Product Name Alternative
Ubiquitin C-terminal hydrolase L3
Certification
RUO
UniProt
P15374
Source
Produced in E. coli .
Stability
As supplied, the enzyme is stable on ice for several hours. Activity is stable to at least 6 freeze/thaw cycles. (snap freezing in a dry/ice ethanol bath or liquid nitrogen) .
Purity
≥90% (SDS-PAGE)
Molecular Weight
26.2 kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 50mM TRIS, pH 7.6, containing 1mM dithiothreitol (DTT) .
Applications Notes
Useful tool to study UCH-L3 kinetics and regulation, screen for potential inhibitors and activators.
GenBank Number
NM_006002
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