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Ubiquitin, (biotinylated)

Product Specifications

Certification

RUO

UniProt

P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)

Source

Produced in E. coli.

Purification

Mass spectral analysis shows presence of [biotinyl]1-5-ubiquitin with [biotinyl]2-4-ubiquitin being the predominate species.

Stability

As indicated on product label or CoA when stored as recommended.

Solubility

In order to ensure full solubility it is recommended that the material be dissolved in 5μL DMSO prior to dilution with the buffer of choice to the desired working concentration.

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Appearance

White solid.

Formulation

Lyophilized from Acetonitrile:water (1:1) .

Applications Notes

Biotinylated ubiquitin is activated by E1 and is a competent substrate for E2 and E3 enzymes. The biotinyl moiety also provides a potential route to affinity purification of labeled species.

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