Ubiquitin, (biotinylated)
Product Specifications
Certification
RUO
UniProt
P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
Source
Produced in E. coli.
Purification
Mass spectral analysis shows presence of [biotinyl]1-5-ubiquitin with [biotinyl]2-4-ubiquitin being the predominate species.
Stability
As indicated on product label or CoA when stored as recommended.
Solubility
In order to ensure full solubility it is recommended that the material be dissolved in 5μL DMSO prior to dilution with the buffer of choice to the desired working concentration.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Appearance
White solid.
Formulation
Lyophilized from Acetonitrile:water (1:1) .
Applications Notes
Biotinylated ubiquitin is activated by E1 and is a competent substrate for E2 and E3 enzymes. The biotinyl moiety also provides a potential route to affinity purification of labeled species.
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