Ubiquitin monoclonal antibody (P4D1)

Product Specifications

Certification

RUO

UniProt

P0CG53 (UBB), P0CH28 (UBC), P62992 (RPS27A), P63048 (UBA52)

Host

Mouse

Species Reactivity

Species independent

Immunogen

Denatured bovine ubiquitin.

Clone

P4D1

Source

Purified from hybridoma tissue culture supernatant.

Applications

IHC, IP, WB

Purification

Protein G affinity purified form cell culture media.

Stability

Stable for at least 1 year after receipt when stored at -20°C.

Dilution

Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

The ubiquitin antibody BML-PW0930 (clone P4D1) was developed using a denatured glutaraldehyde cross-linked ubiquitin IgG complex as the immunogen. Monoclonal antibody was purified from tissue culture supernatant by ion exchange chromatography. The general ubiquitin reactivity of the monoclonal antibody has been demonstrated by Western blotting using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in cell lysates.

Specificity

Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin.

Formulation

Liquid. In PBS containing 0.01% sodium azide.

Applications Notes

Clone P4D1 has been reported to be of utility in immunoprecipitation, immunofluorescence and immunohistochemistry (see Brady et al. (2005) ; Wang et al. (2008) ; Ito et al. (2003) respectively) .