Ubiquitin conjugating kit (HeLa lysate-based)

All kit components should be stored at -80°C to ensure stability and activity. Inhibitors are stable for up to 3 months at -20°C when dissolved in DMSO.

Uses: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments.

HeLa S100 fraction (Prod. No. BML-SW8750), 1 mg (200μl provided as 2x100μl vials), 5mg/ml 10x Ubiquitin solution (UB) (Prod. No. BML-UW8795), 100μl (provided as 2X50μl vials), 1mg/ml 10x ATP- (Energy) regeneration solutions (ATP-ERS) (Prod. No. BML-EW9810), 100μl 10x Ubiquitinylation buffer (Prod. No. BML-KW9885), 100μl Ubiquitin aldehyde (inhibitor) (Prod. No. BML-UW8450), 50 μL, 1mg/ml Ubiquitin-conjugate specific antibody solution (UBCJ2) (Prod. No. ENZ-ABS840), 10 μl, Mono- and polyubiquitinylated conjugates, mAb