Ubiquitin activating kit

Uses: Activation of Ubiquitin for conjugation to wild type or mutant E2 enzymes (user supplied) via thioester bond formation. Use of Ub-E2 conjugates produced in the subsequent ubiquitin modification of specific target proteins in presence of a dedicated ubiquitin E3 ligase. Investigation of ubiquitin activation by E1 activating enzyme. Substitution of the wild type ubiquitin provided with ubiquitin mutants or derivatives (e.g. biotinylated-ubiquitin, Prod. No. BML-UW8705) allowing their activation and subsequent utility in the ubiquitin cascade.

20X Ubiquitin Activating Enzyme Solution (E1) 20X Ubiquitin Solution (Ub) Ubiquitin Antibody Solution 20X Mg-ATP Solution 2X Non-reducing Gel Loading Buffer 10X Ubiquitinylation Buffer