Ubiquitin (bovine), (native) (methylated)

Product Specifications

Certification

RUO

UniProt

P0CG53

Source

Native bovine.

Stability

As indicated on product label or CoA when stored as recommended. Store solid cold and dry at -20C; stable for at least one year. Store solutions at -20C for up to three months. The protein is stable to multiple freeze-thaw cycles.

Purity

≥95% (SDS-PAGE)

Solubility

Soluble and stable in DMSO and aqueous buffers up to 100 mg/mL. The solid will dissolve with gentle vortexing at the desired concentration.

Molecular Weight

~8.7kDa

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Appearance

White solid.

Formulation

Lyophilized in HPLC-grade water.

Applications Notes

Simplifies kinetics for E1-catalyzed active thioester formation. Typical concentrations for non-rate limiting support of in vitro conjugation reactions lie in the range 200μM to 1mM depending upon individual conditions.