UbcH5c (human), (recombinant) (His-tag)
Product Specifications
Certification
RUO
UniProt
P61077
Source
Produced in E. coli.
Purity
≥90% (SDS-PAGE)
Activity
The His6-tagged fusion proteins of UbcH5a, UbcH5b and UbcH5c all charge and support ubiquitinylation in vitro . Unlike their GST-tagged counterparts, the His 6 -tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.
Molecular Weight
~16kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT.
Applications Notes
Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
PKSV030479 50 µg
Recombinant SARS-CoV-2 Spike RBD Protein (C-6His-Avi) (Omicron), Biotinylated
Sign In for Pricing
View Details 
BNC683736-100 1x 100 µL
Vimentin (Mesenchymal Cell Marker) (VIM/3736), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details