UbcH5c (human), (recombinant) (His-tag)
Product Specifications
Certification
RUO
UniProt
P61077
Source
Produced in E. coli.
Purity
≥90% (SDS-PAGE)
Activity
The His6-tagged fusion proteins of UbcH5a, UbcH5b and UbcH5c all charge and support ubiquitinylation in vitro . Unlike their GST-tagged counterparts, the His 6 -tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.
Molecular Weight
~16kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT.
Applications Notes
Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions.
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