UbcH5b (human), (recombinant) (His-tag)

Product Specifications

Certification

RUO

UniProt

P62837

Source

Produced in E. coli.

Stability

Enzyme is stable to multiple freeze/thaw cycles.

Purity

≥90% (SDS-PAGE)

Activity

The His6-tagged fusion proteins of UbcH5a, UbcH5b and UbcH5c all charge and support ubiquitinylation in vitro . Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The [C 85 A] mutation (Prod. No. BML-UW9065) completely abolishes the ability of the enzyme to form a thiol ester and thereby acts as an excellent negative control.

Molecular Weight

~18kDa

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM NaCl and 0.5mM DTT.

Applications Notes

Useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions.