UbcH5b (human), (recombinant) (His-tag)
Product Specifications
Certification
RUO
UniProt
P62837
Source
Produced in E. coli.
Stability
Enzyme is stable to multiple freeze/thaw cycles.
Purity
≥90% (SDS-PAGE)
Activity
The His6-tagged fusion proteins of UbcH5a, UbcH5b and UbcH5c all charge and support ubiquitinylation in vitro . Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The [C 85 A] mutation (Prod. No. BML-UW9065) completely abolishes the ability of the enzyme to form a thiol ester and thereby acts as an excellent negative control.
Molecular Weight
~18kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM NaCl and 0.5mM DTT.
Applications Notes
Useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions.
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