UbcH2 (human), (recombinant) (His-tag)
Product Specifications
Certification
RUO
UniProt
P62256
Source
Produced in E. coli .
Stability
Enzyme is stable to multiple freeze/thaw cycles.
Purity
≥95% (SDS-PAGE)
Activity
Recombinant UbcH2 charges and supports ubiquitinylation in vitro .
Molecular Weight
~22.2kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 20 mM Tris-HCl, pH 7.5, containing 0.5mM DTT.
Applications Notes
Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions.
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