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UbcH2 (human), (recombinant) (His-tag)

Product Specifications

Certification

RUO

UniProt

P62256

Source

Produced in E. coli .

Stability

Enzyme is stable to multiple freeze/thaw cycles.

Purity

≥95% (SDS-PAGE)

Activity

Recombinant UbcH2 charges and supports ubiquitinylation in vitro .

Molecular Weight

~22.2kDa

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Liquid. In 20 mM Tris-HCl, pH 7.5, containing 0.5mM DTT.

Applications Notes

Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions.

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