TWEAK (soluble) (human), (recombinant)

Apo-3L, TNFSF 12

RUO

O43508

Produced in E. coli . The extracellular domain of human TWEAK (aa 106-249) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG ® -tag.

Stable for at least 6 months after receipt when stored at -20°C.

0.1 mg/mL after reconstitution.

<0.1EU/μg purified protein (LAL test; Bio Whittaker) .

≥90% (SDS-PAGE)

Kills TWEAK sensitive cells.

Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

~18-20kDa (SDS-PAGE) .

Blue Ice

-20°C

Binds to human and mouse Fn14 (TWEAK-R) .

Lyophilized. Contains PBS.

ELISA: binds to FN14 receptor at 10-100 ng/ml.