TRAIL, soluble (human) (recombinant) set
Product Specifications
Specifications
ED50: 10ng/ml (Jurkat cells)
Product Name Alternative
Apo-2L, TNFSF 10, CD253
Certification
RUO
UniProt
P50591
Source
Produced in E. coli . The extracellular domain of human TRAIL (Apo-2L) (aa 95-281) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG ® -tag.
Applications
FUNC
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
TRAIL: 0.1 mg/mL after reconstitutionEnhancer: 1 mg/mL after reconstitution
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute TRAIL with 100μl and each vial of enhancer with 50μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
TRAIL binds to human and mouse TRAIL receptors and human osteoprotegerin (OPG) .
Formulation
Lyophilized. Contains PBS.
Applications Notes
Induces apoptosis in a concentration range of 1-100ng/ml if applied with the cross-linking enhancer.
Contents
10μg of TRAIL, Soluble (human) (recombinant) (Prod. No. ALX-522-003) and 2x50μg of Enhancer for Ligands (Prod. No. ALX-804-034) .
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