TRAIL (soluble) (human), (recombinant) (His-tag)

Product Specifications

Product Name Alternative

Apo-2L, TNFSF 10, CD253

Certification

RUO

UniProt

P50591

Source

Produced in E. coli . Human TRAIL (aa 114-281) is fused to a His-tag.

Purification

Purified by multi-step chromatography.

Stability

When stored at -80°C, this protein is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the protein, followed by refreezing, could lead to loss of activity.

Endotoxin

<0.1 EU/μg TRAIL, determined using chromogenic Limulus amebocyte lysate (LAL) test.

Purity

≥95% (SDS-PAGE)

Activity

Induces apoptosis in U937 cells at 1μg/ml, within 5 hours. At 0.5-2.0μg/ml, induces apoptosis in various thyroid carcinoma lines within 18 hours. User must determine optimal concentrations and time course for induction. Please note that this protein does not require a cross-linking enhancer to exhibit this potent activity.