TRAIL (soluble) (human), (recombinant)

Product Specifications

Product Name Alternative

Apo-2L, TNFSF 10, CD253

Certification

RUO

UniProt

P50591

Source

Produced in E. coli . The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG ® -tag.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Endotoxin

<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .

Purity

≥90% (SDS-PAGE)

Activity

Induces apoptosis if applied with the cross-linking enhancer (Prod. No. ALX-804-034) ; see also TRAIL, Soluble (human) (recombinant) Set (Prod. No. ALX-850-018) . Note: rhsTRAIL does not significantly induce apoptosis in the absence of an enhancer.

Reconstitution

Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Molecular Weight

~28kDa

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Historical data has shown that Fas induces apoptosis in a concentration range of 1-100ng/ml if applied with the cross-linking enhancer (Prod. No. ALX-804-034) .