TRAIL (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
Apo-2L, TNFSF 10, CD253
Certification
RUO
UniProt
P50591
Source
Produced in E. coli . The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG ® -tag.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥90% (SDS-PAGE)
Activity
Induces apoptosis if applied with the cross-linking enhancer (Prod. No. ALX-804-034) ; see also TRAIL, Soluble (human) (recombinant) Set (Prod. No. ALX-850-018) . Note: rhsTRAIL does not significantly induce apoptosis in the absence of an enhancer.
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Molecular Weight
~28kDa
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data has shown that Fas induces apoptosis in a concentration range of 1-100ng/ml if applied with the cross-linking enhancer (Prod. No. ALX-804-034) .
Specificity
Binds to human and mouse TRAIL receptors and human osteoprotegerin (OPG) .
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to human TRAIL receptors and human osteoprotegerin (OPG) at 10-100 ng/ml.
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