TNF-α (soluble) (mouse), (recombinant)

Product Specifications

Product Name Alternative

Tumor necrosis factor-α, TNFSF 2

Certification

RUO

Gene ID

A002291 (UCSD Signaling Gateway ID)

UniProt

P06804

Source

Produced in E. coli . The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG ® -tag.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

1 mg/mL after reconstitution.

Endotoxin

<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .

Purity

≥90% (SDS-PAGE)

Reconstitution

Reconstitute with 50μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Molecular Weight

~20kDa (SDS-PAGE) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Historical lots have shown that it exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells) . FLAG is a registered trademark of Sigma-Aldrich Co.

Specificity

Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.