TNF-α (soluble) (mouse), (recombinant)
Product Specifications
Product Name Alternative
Tumor necrosis factor-α, TNFSF 2
Certification
RUO
Gene ID
A002291 (UCSD Signaling Gateway ID)
UniProt
P06804
Source
Produced in E. coli . The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG ® -tag.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥90% (SDS-PAGE)
Reconstitution
Reconstitute with 50μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Molecular Weight
~20kDa (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical lots have shown that it exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells) . FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to human TNF-R1 receptor.
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