TNF-α (human), (recombinant) (cell culture grade)
Product Specifications
CAS Number
94948-59-1
Specifications
≥2x10 7 U/mg protein. Determined by standard cell cytotoxicity assay (N. Matthes & M.L. Neale; Lymphokines and Interferons, A Practical Approach; M.J. Clemens et al., eds.; IRL Press, p. 221) .
Product Name Alternative
Tumor necrosis factor-α, TNFSF 2
Certification
RUO
UniProt
P01375
Source
Produced in yeast.
Stability
Lyophilized protein is stable at room temperature for a few weeks.
Purity
≥97% (SDS-PAGE)
Activity
Induces cytolysis of mouse L929 cells in the presence of 1μg/ml Actinomycin D (Prod. No. BML-GR300) (ED 50 ≤0.05ng/ml) .
Reconstitution
A quick spin is recommended followed by reconstitution in distilled, sterile water to a concentration of 0.1-1mg/ml. For further dilution, add a carrier protein to avoid loss of biological activity.
Molecular Weight
~17kDa.
Shipping Conditions
Ambient Temperature
Storage Conditions
-20°C
Formulation
Lyophilized from 0.2μm-filtered solution containing mannitol and trehalose.
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