TNF-R1 (human) monoclonal antibody (H398)

Immunohistochemistry (frozen sections; stains acetone-fixed cryostat sections or cell smears. Recommended for H398 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. H398 may be used at a concentration of 1-0.5mg/ml for cryostat sections) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Not recommended for IHC (PS) . Functional Application: blocks TNF-R signalling. 10μg/ml H398 neutralizes TNF induced cytotoxicity on U937 (100%) . Furthermore, 10μg/ml H398 neutralizes TNF induced MHC expression on K562, Colo 205 and IL2R on YT cells (always 100%) . TNF induced cytotoxicity can be inhibited/neutralized by H398 on MCF-7, HeLa and L929. A working concentration could not be determined, since ascites antibody preparations have been used which exhibit an unspecific cytotoxic effect on the cells. Functional activity via the TNF-R1 is completely inhibited by H398 although on some cells the ligand still binds. Thus, the antibody H398 inhibits signalling via the receptor but not always the binding of TNF.