Thrombopoietin (mouse), (recombinant)
Product Specifications
Product Name Alternative
TPO, Megakaryocyte colony stimulating factor, c-MPL ligand, MGDF
Certification
RUO
UniProt
P40226
Source
Produced in E. coli . Non-glycosylated protein, containing 174 amino acids (which comprise the receptor binding domain) .
Stability
It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Endotoxin
≤1 EU/μg protein measured by kinetic LAL analysis.
Purity
≥95% (Reducing and Non-reducing SDS-PAGE)
Activity
The activity is determined by the dose-dependent stimulation of MO7e cells which is typically observed at concentations <1ng/ml.
Reconstitution
When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Molecular Weight
~18.7kDa
Shipping Conditions
Ambient Temperature
Storage Conditions
-20°C
Appearance
White lyophilized (freeze-dried) powder.
Formulation
Lyophilized. Sterile filtered.
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