SUPERKILLERTRAIL® Protein (soluble) (human), (recombinant)

Product Specifications

Product Name Alternative

TNFSF 10, Apo-2L, CD253

Certification

RUO

UniProt

P50591

Source

Produced in E. coli . The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

Purification

Affinity purified. Sterile filtered.

Stability

Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. In order to retain full activity aliquote concentration must be at least 0.1mg/ml; prepare 20μl aliquots and store at -80°C. Working dilution: 2-100ng/ml. Recommended: Prod. No. ALX-505-005, special storage and dilution buffer.

Concentration

0.5 mg/mL

Endotoxin

<0.01EU/μg purified protein (LAL test) .

Purity

≥98% (SDS-PAGE) . MS analysis, <1% impurity (mainly Hsp70 protein from E. coli)

Activity

Induces apoptosis at concentrations of >5ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. Shows superior activity on human cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat) .