SUPERFASLIGAND®Protein (soluble) (human), (recombinant)

Product Specifications

Product Name Alternative

FasL (oligomer), APO-1L (oligomer), CD95L (oligomer), CD178 (oligomer), TNFSF 6 (oligomer)

Certification

RUO

UniProt

P48023

Source

Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG ® -tag. Glycosylation of rhs SUPERFASLIGAND ® is similar to natural human FasL.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Purity

≥95% (SDS-PAGE)

Activity

Kills Fas-sensitive cells. Note: Does not require enhancer.

Reconstitution

Reconstitute with 50μl sterile water. Further dilutions should be made with cell culture medium containing 5% fetal calf serum.

Molecular Weight

~32kDa (nonglycosylated), ~35kDa (glycosylated) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Historical data has shown that SUPERFASLIGAND ® kills Fas-sensitive cells at concentrations of >1ng/ml without the use of enhancer. The ED 50 has been shown in previous data to be 1ng/ml (A20 cells) . Note: Results using rhsSUPERFASLIGAND ® may differ from those obtained with agonistic antibodies! FLAG is a registered trademark of Sigma-Aldrich Co.