SUMO-QAPTURE-T®kit

Unopened kit should be stored at -80°C upon receipt. After thawing, SUMO-QAPTURE-T® matrix should be stored at +4°C. Avoid a second freeze/thaw cycle. SUMO-1, SUMO-2 and SUMO-3 controls should be stored at -80°C. SUMO antibody solutions can be stored at -20°C. Avoid multiple freeze/thaw cycles of components to ensure stability and activity.

For the isolation and enrichment of SUMOylated proteins. Suggested Applications: Capture and isolation of SUMO-protein conjugates from specific cell/tissue lysates of interest with subsequent detection and analysis by Western blotting. Identification of SUMO-modified protein substrates by proteomic analysis methods following release of free SUMOylated proteins in denatured form. Selective purification/pull down of SUMOylated proteins from in vitro SUMOylation assays.

SUMO-QAPTURE® matrix (BML-UW0980-0200) SUMO-1 Control (BML-UW9195-0125) SUMO-2 Control (BML-UW9205-0125) SUMO-3 Control (BML-UW9215-0125) SUMO antibody solutions includes, SUMO-1 polyclonal antibody (BML-PW0505A-0010) SUMO-2 polyclonal antibody (BML-PW0510A-0010)