SUMO-2/3 (human) (NT) polyclonal antibody

Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

The antiserum to SUMO-2 was raised in New Zealand White rabbits to a synthetic peptide corresponding to amino acid residues 1-15 of human SUMO-2. The antibody has been partially purified by salt precipitation and is suspended in PBS containing 0.01% sodium azide as a preservative. BML-PW9465 has been characterised for specificity against various members of the ubiquitin-like protein family including ubiquitin, multi-ubiquitin chains, SUMO-1, UCRP, Nedd8, and FAT10. This antibody shows no cross-reactivity with any of these Ubl proteins upon Western blotting under the conditions tested. Reactivity of the antibody with both pro- and mature forms of SUMO-2 is completely abolished by preincubation of the antibody with its cognate peptide (product code BML-PP9480) . For Western blot an initial dilution of at least 1:1000 is recommended. BML-PW9465 has not been characterised for use in immunoprecipitation or immunohistochemical applications.