SUMO-2 aldehyde

As indicated on product label or CoA when stored as recommended. Soluble and stable in aqueous solution at pH ≤7.0. Avoid presence of amino-containing compounds. Do not lyophilize. Do not neutralize until immediately prior to use. Stable for at least 2 months after receipt when stored at +4°C.

ATTENTION: There is confusion within the scientific literature (including NCBI and UniProt protein databases) concerning the nomenclature used for SUMO2 and SUMO3 paralogs. Please note that Enzo Life Sciences uses the nomenclature proposed by Saitoh and Hinchey [J. Biol. Chem, 275 , 6252-6258 (2000) ] for SUMO-2/SMT3A and SUMO-3/SMT3B and reports data accordingly.

Suggested uses: Stabilisation of SUMO-protein conjugates in cell lysates and tissue extracts. Determination of inhibition kinetics for specific deSUMOylating enzymes. Co-crystallisation with specific deSUMOylating enzymes for structural studies Probe for investigating enzyme:substrate interactions. Used in conjunction with SUMO-1 aldehyde to probe the SUMO paralogue preference of SENP enzymes

SUMO-2 aldehyde activity was confirmed by its inhibition of SENP1 (Prod. No. BML-UW9760) deSUMOylation of SUMO2-AMC (Prod. No. BML-UW0045) . Typical assay set-up: Substrate concentration: 0.1-2.0μM. Enzyme concentration: 0.1-1μM. Inhibitor concentration: 0.1-2.0μM. Release of AMC fluorescence by DUB enzymes can be monitored spectrophotometrically using 380nm excitation and 460nm emission wavelengths. Concentration determination: SUMO-2 aldehyde sample concentration determined by measurement of absorbance at 280nm by spectrophotometry, and comparison with a SUMO-1 calibration curve produced by serial dilution (2.0-0.125mg/ml) .