SUMO-1 (93-97) -AMCA
Product Specifications
Certification
RUO
Sequence
E 93 QTGG 97 -AMCA
Stability
As indicated on product label or CoA when stored as recommended. Store solid at -20°C for up to twelve months. Solutions can be stored for up to three months at -20°C.
Purity
≥95% (HPLC)
Solubility
Soluble in DMSO. Can be subsequently diluted in aqueous buffers.
Molecular Weight
747
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Formulation
Lyophilized solid.
Applications Notes
Useful fluorogenic substrate for deSUMOylating enzymes. Typical assay conditions: Assay substrate concentration: 12.5μM-100μM. Enzyme concentrations: 0.5μM-4μM. Release of AMCA fluorescence by deSUMOylating enzymes can be monitored using fluorimetry (380nm excitation and 460nm emission wavelengths) . Uses: Substrate for deSUMOylating enzyme activity assays. Identification/confirmation of enzyme deSUMOylation activity. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC/AMCA substrates (e.g. SUMO1-AMC, NEDD8-AMCA) .
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