Stem cell factor (human), (recombinant)
Product Specifications
Product Name Alternative
SCF, c-Kit ligand, KL, Steel factor, MGF
Certification
RUO
UniProt
P21583
Source
Produced in E. coli . Non-glycosylated protein, containing 165 amino acids.
Stability
It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Endotoxin
≤1 EU/μg protein measured by kinetic LAL analysis.
Purity
≥97% (SDS-PAGE using Coomassie Staining)
Activity
The activity is determined by the dose-dependent stimulation of human TF-1 cells which is typically observed at a concentration of ≤ 15 ng/mL.
Reconstitution
When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Molecular Weight
~18.4kDa
Shipping Conditions
Ambient Temperature
Storage Conditions
-20°C
Appearance
White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 μm filtered solution containing 10 mM Sodium Phosphate, 50 mM Sodium Chloride, pH 7.5.
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