ROS-ID®Total ROS detection kit

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

The ROS-ID ® Total ROS detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. Application Note: Image-Based Analysis of a Human Neurosphere Stem Cell Model for the Evaluation of Potential Neurotoxicants

This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.

A sample from each lot of ROS-ID ® Total ROS detection kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard green filter (490/525 nm) . The following results are obtained: ROS positive control samples induced with Pyocyanin exhibit bright green fluorescence in the cytoplasm. Cells pretreated with the ROS inhibitor don’t demonstrate any green fluorescence signal upon induction.

Oxidative Stress Detection Reagent (Green), 300 nmoles ROS Inducer (Pyocyanin), 1 μmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack