ROS-ID®ROS/RNS detection kit

With proper storage, the kit components are stable up to the date noted on the product label. Store the kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

The ROS-ID ® ROS/RNS detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy.

ROS-ID ® ROS/RNS detection kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard green (490/525 nm), orange (550/620 nm), and red (650/670 nm) fluorescent cubes. Stained HeLa cells induced with L-arginine exhibit bright red fluorescence with a red punctuate cytoplasmic staining pattern. L-arginine-induced cells incubated with NO Scavenger (c-PTIO) demonstrate minimal red fluorescence comparable to control values. Stained HeLa cells induced with pyocyanin exhibit bright orange signal in the nucleus as well as bright green fluorescence in the cytoplasm, but no red signal specific for nitric oxide. Pyocyanin-induced cells incubated with ROS Inhibitor (N-acetyl-L-cysteine) demonstrate minimal green or orange fluorescence signal comparable to control values.

NO Detection Reagent (Red), 60μl Oxidative Stress Detection Reagent (Green), 300 nmoles Superoxide Detection Reagent (Orange), 300 nmoles NO Inducer (L-Arginine), 100 μl ROS Inducer (Pyocyanin), 1 μmole NO Scavenger (c-PTIO), 400 nmoles ROS Inhibitor (N-acetyl-L-cysteine), 2x10 mg 10X Wash Buffer, 15 ml