Renin (human), (recombinant) (active)
Product Specifications
Specifications
≥30U/μg. 1 unit is defined as the amount of enzyme that cleaves 1 pmole of the fluorogenic peptide substrate (Renin Substrate 1), Arg-Glu (EDANS) -Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys (DABCYL) -Arg.
Product Name Alternative
Angiotensinogenase, Angiotensin forming enzyme, Ren
Certification
RUO
UniProt
P00797
Source
Produced in HEK cells. Mature Renin (aa 67-406) is fused at the N-terminus to a FLAG ® -tag. Active form, does not contain prorenin peptide (aa 1-66) .
Purification
Affinity purifed.
Stability
Reconstitute in 100μl deionized H2O for a final concentration of 0.1mg/ml.
Concentration
0.1 mg/mLwhen reconstituted as suggested.
Endotoxin
<0.1EU/μg protein (LAL test; Associates of Cape Cod) .
Purity
≥90%
Molecular Weight
~52kDa (predicted is 38.3 kDa)
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
FLAG ® is a registered trademark of Sigma-Aldrich Co.
Formulation
Lyophilized. In 25mM MES buffer containing 150mM NaCl.
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