Red hydrogen peroxide assay kit

This product is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

A sample kit from each lot of Red Hydrogen Peroxide Assay Kit is used to detect H 2 O 2 using the protocols described in the user manual. Minimum Specifications: 1. A minimum of 10 picomoles of H 2 O 2 must be detected in solution under normal conditions. 2. Fluorescence-based detection must yield H 2 O 2 specific excitation and emission signals at 570 nm and 585 nm, respectively. 3. Absorbance-based detection must yield an H 2 O 2 specific signal at OD 576 .

Red Peroxidase Substrate (1 vial) Horseradish Peroxidase (HRP) (20 units) Hydrogen Peroxide (H 2 O 2) Stock Solution (200 μl) DMSO (1 ml) Assay Buffer (100 ml)