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RANKL (soluble) (human), (recombinant)

Product Specifications

Product Name Alternative

TRANCE, OPGL, ODF, TNFSF 11, CD254

Certification

RUO

UniProt

O14788

Source

Produced in HEK 293 cells. The extracellular domain of human RANKL (aa 152-317) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG ® -tag.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Endotoxin

<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .

Purity

≥90% (SDS-PAGE)

Reconstitution

Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Solubility

Soluble in water.

Molecular Weight

~18kDa (non-glycosylated), ~28kDa (glycosylated) (SDS-PAGE) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

FLAG is a registered trademark of Sigma-Aldrich Co.

Specificity

Binds to human and mouse RANK.

Formulation

Lyophilized. Contains PBS.

Applications Notes

ELISA: binds to RANK receptor.

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