RANKL (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
TRANCE, OPGL, ODF, TNFSF 11, CD254
Certification
RUO
UniProt
O14788
Source
Produced in HEK 293 cells. The extracellular domain of human RANKL (aa 152-317) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG ® -tag.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥90% (SDS-PAGE)
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Solubility
Soluble in water.
Molecular Weight
~18kDa (non-glycosylated), ~28kDa (glycosylated) (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
Binds to human and mouse RANK.
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to RANK receptor.
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