PTP1B drug discovery kit

Store all components except the microtiter plate at -70°C for the highest stability. The PTP1B enzyme component BML-SE332-9092 must be handled particularly carefully in order to retain maximal enzymatic activity. Thaw it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be ‘snap’ frozen, e.g. in liquid nitrogen or a dryice/ethanol bath, and stored at -70°C. If only a few assays are to be performed each day, the PTP1B may be divided into several aliquots (best if ≥ μl) ) to help minimize freeze/thaw cycles.

PTP 1B Enzyme, Substrate (“IR5” Insulin Receptor β, residues 1142-1153, pY 1146), Assay Buffer, Biomol RedTM Concentrated Phosphate Detection Reagent, Suramin (PTP1B inhibitor), Phosphate Standard, and Microplate (1⁄2-volume)