PROTEOSTAT®Protein refolding and aggregation sensing kit

The PROTEOSTAT ® Protein refolding and aggregation sensing kit is a member of the PROTEOSTAT ® product line, reagents and assay kits that have been extensively tested in protein aggregation applications. PROTEOSTAT ® reagents and kits are optimal for demanding protein analyses involving aggregation using microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.

PROTEOSTAT ® Protein refolding and aggregation sensing kit provides a fractional factorial matrix design to facilitate screening of refolding parameters for a specific protein in different buffers, and a simple, homogenous assay format for monitoring protein aggregation using a red-emitting aggregation-sensitive dye.

PROTEOSTAT ® Detection Reagent , 22 μl 10X PROTEOSTAT ® Assay Buffer , 1 x 2 ml 2X Refolding Buffers 1-15 , 10 ml each Lysozyme standard , 10 mg Denaturant : 6M Guanidine Hydrochloride (GdnHCl), Tris-HCl (pH 8), 14 ml 100 mM DTT , 1 ml GSH , 46.1 mg GSSG , 18.4 mg 10 mM PEG , 1 ml 500 mM EDTA , 1 ml 400 mM CaCl 2 , 1 ml 400 mM MgCl 2 , 1 ml 1 M NaCl , 1 ml 0.1% Tween-20 , 1 ml 1.2 M Sucrose , 1 ml α-Cyclodextrin (200 mg/ml), 1 ml