PROTEOSTAT®Protein refolding and aggregation sensing kit

Product Specifications

Certification

RUO

Stability

With proper storage, the kit components are stable for one year from date of receipt.

Shipping Conditions

Dry Ice

Storage Conditions

-20°C

Notes

The PROTEOSTAT ® Protein refolding and aggregation sensing kit is a member of the PROTEOSTAT ® product line, reagents and assay kits that have been extensively tested in protein aggregation applications. PROTEOSTAT ® reagents and kits are optimal for demanding protein analyses involving aggregation using microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.

Applications Notes

PROTEOSTAT ® Protein refolding and aggregation sensing kit provides a fractional factorial matrix design to facilitate screening of refolding parameters for a specific protein in different buffers, and a simple, homogenous assay format for monitoring protein aggregation using a red-emitting aggregation-sensitive dye.

Quality Control

Using the procedure described in the manual, denatured lysozyme is refolded to compare the relative fluorescence in different buffers.

PROTEOSTAT ® Detection Reagent , 22 μl 10X PROTEOSTAT ® Assay Buffer , 1 x 2 ml 2X Refolding Buffers 1-15 , 10 ml each Lysozyme standard , 10 mg Denaturant : 6M Guanidine Hydrochloride (GdnHCl), Tris-HCl (pH 8), 14 ml 100 mM DTT , 1 ml GSH , 46.1 mg GSSG , 18.4 mg 10 mM PEG , 1 ml 500 mM EDTA , 1 ml 400 mM CaCl 2 , 1 ml 400 mM MgCl 2 , 1 ml 1 M NaCl , 1 ml 0.1% Tween-20 , 1 ml 1.2 M Sucrose , 1 ml α-Cyclodextrin (200 mg/ml), 1 ml