PROTEOSTAT®Protein aggregation standards

Application Notes: Particle analysis of therapeutic protein formulations with ImageStreamX ® Imaging Flow Cytometry and the PROTEOSTAT ® Protein Aggregation Assay Prediction of Aggregation Propensity and Monitoring of Aggregation of Antibody-Drug Conjugates (ADC) using ProteoStat ® Reagents This product is manufactured and sold by ENZO LIFE SCIENCES, INC. for research use only by the end-user in the research market and is not intended for diagnostic or therapeutic use. Purchase does not include any right or license to use, develop or otherwise exploit this product commercially. Any commercial use, development or exploitation of this product or development using this product without the express prior written authorization of ENZO LIFE SCIENCES, INC. is strictly prohibited.

This kit is ideal for reliable and accurate quantification of protein aggregation in solution. The standards are used to determine low levels of aggregated protein in a sample by comparing the assay response of a test sample to that of the standard curve; comprised of standards with known concentrations of aggregated IgG.

A sample from each lot of PROTEOSTAT ® Protein Aggregation Standards is used with the procedures described in the user manual. Perform assay with 12.5% – 0% Aggregate in duplicate. Linear curve R-Square has to be greater than 0.95.

A. PROTEOSTAT ® Control (0.0% aggregate) B. PROTEOSTAT ® Control (0.20% aggregate) C. PROTEOSTAT ® Control (0.39% aggregate) D. PROTEOSTAT ® Control (0.78% aggregate) E. PROTEOSTAT ® Control (1.56% aggregate) F. PROTEOSTAT ® Control (3.13% aggregate) G. PROTEOSTAT ® Control (6.25% aggregate) H. PROTEOSTAT ® Control (12.5% aggregate) Deionized Water , 5 ml