Proteinase inhibitor 9 (human) monoclonal antibody (PI9-17)
Selected Variant
ALX-804-457-C125
Size: 125 µg
Product Specifications
Product Name Alternative
PI9, Serpin B9, CAP3, Cytoplasmic antiproteinase 3
Certification
RUO
UniProt
P50453
Host
Mouse
Species Reactivity
Human
Immunogen
Recombinant human full-length PI9 (proteinase inhibitor 9) .
Clone
PI9-17
Applications
IHC (PS), WB
Positive Control
YT-Indy cells (a natural killer leukemia cell line) can be used as positive control. In other cell lines a non-specific protein band of ~63kDa can be detected.
Dilution
Immunohistochemistry (formaldehyde-fixed paraffin-embedded human tissues after pretreatment with 10mM citric acid pH 6.0 for 20min; use ~1:100. For fixed cytospin preparations fix cells for 10min in 10% formalin and then treat them as paraffin sections) Western Blot (standard procedures) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Formulation
Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
Applications Notes
Detects bands of ~42kDa (native PI9) and ~67kDa (PI9 complexed to granzyme B) by Western blot. Not suitable for Flow Cytometry.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgG1
Cross Reactivity Details
Does not cross-react with PI6, PI8 or PAI-2.
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