Protein A ELISA kit (Flexible Protocol)
Product Specifications
Certification
RUO
Species Reactivity
Species independent
Applications
Colorimetric detection, ELISA
Wavelength
450 nm
Sensitivity
9.01 pg/ml (range 15.63 – 1,000 pg/ml)
Compatibility
This product is compatible with the Absorbance 96 Plate Reader .
Shipping Conditions
Blue Ice
Storage Conditions
+4°C
Notes
Assay recognition of different Protein A constructs from natural Protein A from S. aureus (M), recombinant Protein A from E. coli (R), recombinant Cys-Protein A from E. coli (G), and recombinant alkaline-resistant Protein A variant from E. coli (S) were tested and the resulting concentrations were interpolated from kit standard curve and displayed in the figure provided. Application Notes: Comparison of Assay Methods for the Detection of Residual Protein A in Biological Therapeutics
Applications Notes
For the quantitative determination of residual protein A contamination in protein A purified IgG preparations from any species.
Contents
Microtiter plate, Conjugate, Antibody, Dissociation buffer, Neutralization buffer, Assay buffer 13, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
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